IN-VITRO EFFECTS OF HESPERIDIN ON HUMAN ERYTHROCYTES SICKLING WITH MODIFICATIONS OF FUNCTIONAL CHEMISTRY

Sickle cell disease (SCD) is still one of the highest leading inbred hemoglobinopathy amid Africans. The In-vitro effects hesperidin on sickling human erythrocytes with functional chemistry sickle were investigated by standard methods.The induction in was done using 2%sodium metabisulphite at 3 hours optimally. prevention and curative approaches (PA CA) adopted comparison erythrocytesfrom AA-genotype. grouped into 3; AA–hesperidin treated (AA××), (AA×) serving as negative control (NC). results showed 83% 86% antisickling potency for respectively. analysis that modifies groups favor its judging from FTIR observed bends shifts. From GC-MS LC-MS analyses, it practical treatment favors productions fatty acid alkyl monoesters (FAMEs) concomitant shutting down-effects selenocompound metabolism. Pathway enrichment topology analyses revealed non-specifically activation biosynthesis, linoleic metabolism steroid hormone biosynthesis pathways upon treatment. Thus, sickling-suppressive could potentially be associated modulation oxygenated deoxygenated haemglobinthroughpossible modification erythrocyte’s chemistry.